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KMID : 0380619900220070802
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Korean Journal of Food Science and Technology
1990 Volume.22 No. 7 p.802 ~ p.804
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Colorimetric Determination of α - Cyclodextrin by Using the Decoloration of Starch - iodine Complex
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Abstract
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A new colorimetric method to analyze a-cyclodextrin(a-CD) by using a property of α-CD to decolor the starch-iodine complex, was presented. α-CD was quantitatively determined as follows. The α-CD standard solutions at concentrations up to 2 mg/ml were prepared. To 1 ml of starch-iodine complex solution which contained starch(1%) and iodine(0.02% I₂, 0.2% KI) equivalently in distilled water, 200 μl of α-CD standard solutions and 3 ml of distilled water were added and the absorbance was measured at 570 nm. Using this method α-CD concentration range at 02 mg/ml could be determined and their absorbance patterns at 570 nm showed a good linearity. β-CD and glucose had no interference in this method for α-CD determination.
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